Adjuvant induced immunomodulatory effects

In the project, immune modulatory effects of a saponin adjuvant (G3) are studied in the pig and the horse. Saponin adjuvants are used in a number of veterinary vaccines and some are currently in human clinical trials. The project focuses on the early inflammatory response provoked by the adjuvant and is mainly carried out in vitro.

The Section of Immunology at the Department of Biomedicine and Veterinary Public Health, SLU, performs basic research with focus on infection biology with special attention to the interaction of infectious and non-infectious agents with host immune defence parameters. Immune mechanisms are studied at the cellular-, protein- and molecular level.

The present project is based on results from a global transcriptional analysis, using the Affymetrix porcine gene chip. In particular, the activation of interferon-regulated genes (IRGs) and other early cytokines will be analysed. Type I interferon (interferon alpha/beta) is induced by nucleic acids and a synthetic RNA analogue (polyI:C) and ODNs will be used as controls.

The G3 adjuvant will be tested alone or in combination with other immune modulatory compounds such as agonists to toll like receptors (TLRs) or putative vaccine antigens. The cytokine response will be quantified using qPCR in combination with immunoassays. Mononuclear cells and polymorphonuclear leukocytes will be purified from porcine or equine blood and used for the in vitro assays.

If you are interested in the project, please contact:
Caroline Fossum
Dept. of Biomedicine and Veterinary Public Health, SLU
caroline.fossum@slu.se